Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency.
Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed. Cell biological and molecular characteristics of pseudorabies virus infections aujesz,y cell cultures and in pigs with emphasis on the respiratory tract.
Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Articles from Brazilian Journal of Microbiology are ej here courtesy of Elsevier. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
World Organization for Animal Health. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided. Iowa State University Press; The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction. The annealing temperature and number of cycles were determined experimentally.
The virus suinoss replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. Seven virus-negative tissues samples from clinically healthy animals were also included. Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine.
The trigeminal ganglion is the most qujeszky site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect dpena genome present in this site.
PCR experiments were performed on serial ten-fold dilutions of a viral suspension of PRV isolate V with a titer of 10 6. Finally, nucleic auneszky from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
PRV specific primers were designed doenq the Oligo 6. This assay was based on the amplification of a highly conserved viral gD gene fragment. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals.
This region was highly conserved for all reported genomes as shown by aligning of these sequences. The analysis directly from clinical samples from naturally infected animals proved the auinos usefulness of the method for a rapid disease diagnosis from field cases.
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Lanes 1 and 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. National Center for Biotechnology SkinosU. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
The analytical sensitivity of the test was consistently observed to be 1. The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.
This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome The development, optimization and evaluation of a polymerase chain reaction PCR assay are presented for the diagnosis of pseudorabies infection. Negative controls were run with each test. Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4.
This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs Journal List Braz J Microbiol v.
Published online Sep 1. Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes.
Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR.
The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection.
The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products. Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases Can J Com Med.
Doença de Aujeszky
The polymerase chain dd PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 there is no standard procedure recommended so far 2. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.
The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs.